4 research outputs found
Environmental Policy Update 2012: Development Strategies and Environmental Policy in East Africa
The seven chapters that comprise this report explore ways to integrate sustainability goals and objectives into Ethiopia's current development strategies
Three Decades of the Journal of Financial Counseling and Planning
This editorial describes the current status and trends in the past three decades (1990–2019) of the Journal of Financial Counseling and Planning (JFCP). Since its first issue published in 1990, JFCP has become a major research outlet in consumer finance. The journal publishes cutting-edge, peer-reviewed, original research papers on consumer financial counseling, planning, and education that have broad impacts on both academic research and business practices in the field of consumer finance. It is included in many major indexes such as Scopus, Emerging Source Citation Index, EconLit, among others. It has published influential papers on consumer financial well-being, financial capability, financial education, financial counseling, financial planning, retirement planning, risk tolerance, and financial behavior change
Separation and Analysis of Lactosylceramide, Galabiosylceramide, and Globotriaosylceramide by LC-MS/MS in Urine of Fabry Disease Patients
Fabry
disease is an X-linked lysosomal storage disorder caused
by α-galactosidase A (α-GAL A) deficiency. This enzyme
contributes to the cellular recycling of glycosphingolipids such as
galabiosylceramide (Ga<sub>2</sub>), globotriaosylceramide (Gb<sub>3</sub>), and globotriaosylsphingosine (lyso-Gb<sub>3</sub>) by hydrolyzing
the terminal α-galactosyl moiety. Urine and plasma α-GAL
A substrates are currently analyzed as biomarkers for the detection,
monitoring, and follow-up of Fabry disease patients. The sensitivity
of the analysis of Ga<sub>2</sub> is decreased by the co-analysis
of its structural isomer, lactosylceramide (LacCer), which is not
an α-GAL A substrate. A normal-phase ultraperformance liquid
chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) methodology,
allowing the baseline separation of 12 Ga<sub>2</sub> isoforms/analogues
from their lactosylceramide counterparts, was developed and validated
in urine. The method was multiplexed with the analysis of 12 Gb<sub>3</sub> isoforms/analogues having the same fatty acid moieties as
those of Ga<sub>2</sub> for comparison, and with creatinine for sample
normalization. Urine samples were studied from 34 untreated and 33
Fabry males treated by enzyme replacement therapy (ERT) and 54 untreated
and 19 ERT-treated Fabry females, along with 34 male and 25 female
healthy controls. The chromatographic separation of Ga<sub>2</sub> from LacCer increased the sensitivity of analysis, especially in
women. One untreated Fabry female and two treated Fabry females presented
abnormal levels of Ga<sub>2</sub> but normal levels of Gb<sub>3</sub>, supporting the importance of analyzing Ga<sub>2,</sub> in addition
to Gb<sub>3</sub>. Our results show that urine LacCer levels from
females were significantly higher than those from males. Moreover,
LacCer levels were not affected by Fabry disease for both males and
females
Separation and Analysis of Lactosylceramide, Galabiosylceramide, and Globotriaosylceramide by LC-MS/MS in Urine of Fabry Disease Patients
Fabry
disease is an X-linked lysosomal storage disorder caused
by α-galactosidase A (α-GAL A) deficiency. This enzyme
contributes to the cellular recycling of glycosphingolipids such as
galabiosylceramide (Ga<sub>2</sub>), globotriaosylceramide (Gb<sub>3</sub>), and globotriaosylsphingosine (lyso-Gb<sub>3</sub>) by hydrolyzing
the terminal α-galactosyl moiety. Urine and plasma α-GAL
A substrates are currently analyzed as biomarkers for the detection,
monitoring, and follow-up of Fabry disease patients. The sensitivity
of the analysis of Ga<sub>2</sub> is decreased by the co-analysis
of its structural isomer, lactosylceramide (LacCer), which is not
an α-GAL A substrate. A normal-phase ultraperformance liquid
chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) methodology,
allowing the baseline separation of 12 Ga<sub>2</sub> isoforms/analogues
from their lactosylceramide counterparts, was developed and validated
in urine. The method was multiplexed with the analysis of 12 Gb<sub>3</sub> isoforms/analogues having the same fatty acid moieties as
those of Ga<sub>2</sub> for comparison, and with creatinine for sample
normalization. Urine samples were studied from 34 untreated and 33
Fabry males treated by enzyme replacement therapy (ERT) and 54 untreated
and 19 ERT-treated Fabry females, along with 34 male and 25 female
healthy controls. The chromatographic separation of Ga<sub>2</sub> from LacCer increased the sensitivity of analysis, especially in
women. One untreated Fabry female and two treated Fabry females presented
abnormal levels of Ga<sub>2</sub> but normal levels of Gb<sub>3</sub>, supporting the importance of analyzing Ga<sub>2,</sub> in addition
to Gb<sub>3</sub>. Our results show that urine LacCer levels from
females were significantly higher than those from males. Moreover,
LacCer levels were not affected by Fabry disease for both males and
females